Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system

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Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system

Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms. These new platforms, such as 454 and Illumina's MiSeq, have allowed researchers to obtain millions of high quality but short sequences. The result of the added sequencing depth has been significant improv...

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ژورنال

عنوان ژورنال: PeerJ

سال: 2016

ISSN: 2167-8359

DOI: 10.7717/peerj.1869